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A credible Phase I trial would need single and multiple ascending dose pharmacokinetics, tolerability across at least 28 days, off-target enzyme inhibition profiling (especially methyltransferases), and biomarker endpoints such as NNMT activity, SAM/SAH ratio, and NAD+ levels in accessible tissue

As a permanently charged quaternary cation the compound is water-soluble and requires no organic cosolvent for aqueous work, though DMSO stock solutions are common in cell-based screening