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In solvent -80°C, 6 months , -20°C, 1 month All Endogenous Metabolite Isoforms More Biological Activity Description ROS , and alleviates oxidative stress, inflammatory responses, and elevated levels of hepatic enzymes

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2.4 Preparation of paraffin-embedded liver sections Fixed liver tissues (4% PFA, 24 h) were washed with saline and dehydrated using a graded ethanol series (75% → 85% → 90% → 95% → 100% I → 100% II), with each step lasting 0.5–4 h